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Dear Rachel, To my knowledge, alkali metal ions, such as Mg, bind to phosphate groups of nucleic acids.
However, there are other possibilities.
E.g. hydrated magnesium ions are found in the major groove located between G/C base pairs of some oligonucleotides.
On the other, increment in number of Mg ions attached to each oligonucleotide, changes its stacking energy.
At the result, stability of duplex will be increased (It is noteworthy that duplex stability arises from stacking energy, not hydrogenic band!!!).
What is the function of MgCl2 in PCR? - ResearchGate. Available from: https://www.researchgate.net/post/What_is_the_function_of_MgCl2_in_PCR/2 [accessed Oct 7, 2015].
However, there are other possibilities.
E.g. hydrated magnesium ions are found in the major groove located between G/C base pairs of some oligonucleotides.
On the other, increment in number of Mg ions attached to each oligonucleotide, changes its stacking energy.
At the result, stability of duplex will be increased (It is noteworthy that duplex stability arises from stacking energy, not hydrogenic band!!!).
What is the function of MgCl2 in PCR? - ResearchGate. Available from: https://www.researchgate.net/post/What_is_the_function_of_MgCl2_in_PCR/2 [accessed Oct 7, 2015].
