Trả lời mọi thắc mắc của bạn

Liệt kê những bài viết mới nhất

Bài viết mớiNgười viếtVào lúc
paltalk code HdAd
Sun Mar 06, 2016 1:28 am
vb ListView HdAd
Sun Mar 06, 2016 1:00 am
Find occurent of character in string nth HdAd
Sat Jan 16, 2016 10:48 pm
Send listbox items to textbox HdAd
Wed Jan 13, 2016 9:55 pm
[VB] Find word in listbox items HdAd
Wed Jan 13, 2016 7:55 pm
code cho hint2 HdAd
Wed Jan 13, 2016 3:22 pm
[vb] Font RTB HdAd
Tue Dec 29, 2015 10:29 am
[vb] selected text RTB HdAd
Tue Dec 29, 2015 9:06 am
[vb] send unicode text HdAd
Tue Dec 29, 2015 9:03 am
[vb] format multi richtextbox HdAd
Tue Dec 29, 2015 9:01 am
Trang web tai cac bang sang che HdAd
Mon Dec 28, 2015 1:28 pm
[VB] Sử dụng resource của chính chương trình HdAd
Sat Dec 26, 2015 8:56 pm
[vb] Phân biệt class và Module HdAd
Thu Dec 24, 2015 2:39 pm
[VB] MY API and Functions declaration HdAd
Mon Dec 21, 2015 1:45 pm
[vb] Khai báo và sử dụng hàm Setforeground HdAd
Mon Dec 21, 2015 1:29 pm
[VB] Sử dụng hàm Sendmessage để gửi tiếng việt, và format richtextbox HdAd
Mon Dec 21, 2015 1:26 pm
[VB] Find menu context handle/ID by SPY++ HdAd
Fri Dec 18, 2015 1:20 pm
[VB] Find menu context handle/ID - Vu Hai Ninh HdAd
Fri Dec 18, 2015 1:00 pm
[VB] Find menu context handle/ID HdAd
Fri Dec 18, 2015 12:54 pm
[VB] Sleep in VB program HdAd
Thu Dec 17, 2015 7:48 pm
[VB] CLose window if found HdAd
Wed Dec 16, 2015 7:07 pm
[VB] Không cho thay đổi kích cỡ form khi chạy HdAd
Wed Dec 16, 2015 3:28 pm
[VB] Không cho thay đổi text box khi chạy HdAd
Wed Dec 16, 2015 3:22 pm
[VB] Tìm cửa sổ và Button bằng FindWindow & FindWindowsEx HdAd
Wed Dec 16, 2015 3:16 pm
[VB] Xóa text mặc định của text box khi click HdAd
Wed Dec 16, 2015 3:12 pm

You are not connected. Please login or register

Khác biệt giữa EDTA và EGTA là gì?

Go down  Message [Page 1 of 1]

1Khác biệt giữa EDTA và EGTA là gì? Empty Khác biệt giữa EDTA và EGTA là gì? on Fri Sep 25, 2015 8:23 am


1. Cả 2 đều là tác nhân chelating (bọc các ion kim loại trong dung dịch phản ứng lại, để chúng ko tiếp tục ảnh hưởng tới phản ứng/cấu trúc), đều dạng bột tinh thể trắng.

2. EGTA thì hấp dẫn các ion Canxi 2+, trong khi đó EDTA thì hấp dẫn các ion Mg 2+

3. EDTA được dùng nhiều hơn EGTA.

4. EGTA có nhiệt độ sôi và Flashing (ko biết là cái gì) cao hơn EDTA.

5.EDTA đặc hơn EGTA

Muốn biết chi tiết đọc bài ở dưới


Khác biệt giữa EDTA và EGTA là gì? General-pd6

Chemistry can be one of the hardest subjects for students in schools. It seems as though most students have developed a detachment from the subject no matter how teachers explain the importance of chemistry in many industries and fields. Then again, the students wouldn’t find it difficult to appreciate chemistry if they realized how much weight it poses on the advancement of various industries, especially in medicine.
The biggest application of the knowledge acquired from a thorough study and understanding of the chemical processes in the field of medicine is probably the procedures that include ethylenediaminetetraacetic acid or EDTA and or ethylene glycol tetraacetic acid or EGTA.
Both components are used in phlebotomy and in preserving samples of patients’ body fluids. More often than not, nonetheless, EDTA is used more than EGTA. This is because of its ability to bind metal ions which is applicable in the buffering of electrophoresis.
Biologists dedicated to studying DNA and RNA behavior often use EDTA as it is more effective in preventing the enzymes of DNA or RNA from degrading. On theory, the EDTA “freezes” any activity of the enzyme by chelating the magnesium ions which are known to trigger the activeness of the enzymes. Using EDTA does not affect enzyme activity, but it generally halts their natural activity and allows for the requirement of calcium ions to be determined.
The EDTA is also known to have applications in providing an immediate cure to metal poisoning. The food industry also utilizes EDTA as a preservative.
EGTA is as useful as EDTA in phlebotomy. It is known to be a chelating agent like EDTA, but EGTA works through preferentially binding to calcium ions. Most phlebotomists and specialists alike use EGTA to chelate calcium ions under a fully-equipped laboratory during cell-based experiments.
Generally, however, EDTA and EGTA are by nature two similar substances. These two acids are comprised of polyamino carboxylic acids and appear to be white crystalline powders when used in laboratory experiments. Both work by binding certain kinds of molecules. Looking through their chemical makeup, their reactions upon exposure to certain molecules and their applications, nonetheless, can draw their differences.
EGTA being able to bind calcium ions contains more carbon, hydrogen, and oxygen than EDTA. EGTA has 14 atoms of carbon, 24 atoms of hydrogen, 10 atoms of oxygen, and 2 nitrogen atoms. This creates the chemical makeup of EGTA, C14 H24N2O10.

EDTA, on the other hand, only contains 10 atoms of carbon, 16 atoms of hydrogen, 8 atoms of oxygen and 2 nitrogen atoms making its chemical makeup take the form of C10 H16N2O8.
As mentioned earlier, the two acids can be utilized as a chelating agent. Nonetheless, EDTA and EGTA don’t bind the same way. EGTA can be more suitable to be used with a divalent calcium cation. EDTA, on the other hand, is observed to be more attracted to a divalent magnesium cation. Thus, the utilization of these two acids will greatly depend on the substances on which they will be used for lab experiments.
Chemists, phlebotomists, and other scientists have also recorded a higher boiling point of EGTA in comparison to EDTA. At 769 millimeters of mercury (mm. of Hg), EGTA boils at 678 degrees Celsius. With the same exposure to atmospheric pressure, EDTA had been observed to boil only at 614.186 degrees Celsius.
It then follows that EGTA’s flash point is higher than EDTA’s at 363.9 degrees Celsius (for EGTA) compared to a mere 325.247 degrees Celsius (for EDTA). EDTA’s higher density can be accounted for the lower boiling and flashing point. EDTA weighs 1.566 g/cm3 while EGTA only takes on a mass of 1.433 g/cm3.

View user profile

Back to top  Message [Page 1 of 1]

Permissions in this forum:
You cannot reply to topics in this forum